IN VITRO EFFICACY OF VARIOUS CONCENTRATIONS OF COCONUT SHELL LIQUID SMOKE AGAINST FUSARIUM OXYSPORUM F. Sp. LYCOPERSICI

Chairudin Chairudin; Sumeinika  Fitria Lizmah; Agustinur Agustinur; Dwi Purnomo; Irvan  Subandar

Chairudin Chairudin Department of Agrotechnology, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia
Sumeinika Fitria Lizmah Department of Agrotechnology, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia
Agustinur Agustinur Department of Agrotechnology, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia
Dwi Purnomo Department of Agrotechnology, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia
Irvan Subandar Department of Agrotechnology, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia

Keywords: Fusarium oxysporum, Liquid smoke, Concentration, efficacy, in vitro.

Abstract

Fusarium oxysporum F. sp. lycopersici is one of a fungi that causes wilt disease in tomato plants. To overcome this problem is by developing bioactive agents, namely coconut shell liquid smoke, which contains phenolic compounds, organic acids and alcohol that act as antibacterial and antifungal agents. The purpose of this study was to determine the efficacy of coconut shell liquid smoke in inhibiting the growth of F. oxysporum F. Sp. lycopersici in vitro. The design used was a non-factorial completely randomized design (CRD) consisting of 6 treatments and 3 replications with coconut shell liquid smoke factor (A), consisting of 6 levels, namely A0: 0% (Control), A1: 2%, A2: 4%, A3: 6%, A4: 8% and A5: 10%. Parameters observed were colony diameter, percentage of colony relative inhibition, sporulation and growth compatibility of Fusarium oxysporum F. sp. lycopersici with coconut shell liquid smoke. The results showed that coconut shell liquid smoke had a very significant effect on colony diameter and colony relative resistance percentage. Coconut shell liquid smoke had a very significant effect on colony diameter at concentrations of A4 (8%) and A5 (10%), while the percentage of relative inhibition of colonies in A4 and A5 treatments with 90% inhibition. Liquid smoke also affects the sporulation and growth compatibility of Fusarium oxysporum F. sp. lycopersici in treatment A4 (8%) and A5 (10%) each had a percentage of 0 with 100% inhibition.