SOIL ANALYSIS TEST, PHYTOCHEMISTRY, AND ANTI-INFLAMMATORY ABILITY OF PAPAYA LEAF AS AN ERGOGENIC ANTIFATIGUE CANDIDATE IN WISTAR RATS
The main objective of this study was to identify differences in soil nutrient composition, biological activity, and anti-inflammatory properties of papaya leaves at three different locations above sea level. The three locations are in Ie Seum (geothermal area), Kajhu (coastal), and Lambhuk (lowland). The chemical composition of the soil on papaya leaves was analyzed using GC-MS, AAS, a Flamephotometer, and a Spectrophotometer. The highest soil macro and micronutrients were found in the Kajhu area with a pH (H2O) value of 8.05; pH (KCl) 7.15; P 51.35 mg kg-1; Ca++ 13.20 cmol kg-1; K+ 0.99 cmol kg-1; Na+ 0.32 cmol kg-1; cation exchange capacity 21.20 cmol kg-1; base saturation 71.04%. The number of compounds identified from biological activity in Kajhu District was 24 compounds; 23 compounds in Lambhuk District; and 17 compounds in Ie Seum District. The highest compounds contained in all samples were neophytadiene, linolenic acid, gamma, tocopherol, hexadecanoic acid, vitamin e, carpaine, 9,12, and 15 octadecatrienoic acid, norolean-12-ene, squalene, and phytol. From the three locations, it can be concluded that the coastal area of Kajhu contains higher levels of nutrient compounds, biological activity, and anti-inflammatory and antioxidant properties than the other two locations.